Blood levels of lopinavir not related to increase in lipids

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Plasma concentrations of the protease inhibitor lopinavir are not associated with elevated blood lipids, according to an Italian study published in the March 1st edition of the Journal of Acquired Immune Deficiency Syndromes.

Investigators from the on-going Resistance and Dosage Adapted Regimens (RADAR) study into therapeutic drug monitoring conducted the study following suggestions that levels of Kaletra in the blood were associated with a risk of serious (grade three) elevations in total triglyceride and cholesterol levels. On the basis of these data it has been suggested that the standard dose of Kaltera should be reduced to help counter lipid increases.

A total of 55 individuals were recruited to the study. The average age was 39 years, 29 (64%) were men, and 29 individuals were also coinfected with hepatitis C virus, and a further two individuals were coinfected with hepatitis B virus. Plasma concentrations of lopinavir were monitored at entry to the study and then at weeks four and twelve.

Glossary

triglycerides

A blood fat (lipid). High levels are associated with atherosclerosis and are a risk factor for heart disease.

 

plasma

The fluid portion of the blood.

cholesterol

A waxy substance, mostly made by the body and used to produce steroid hormones. High levels can be associated with atherosclerosis. There are two main types of cholesterol: low-density lipoprotein (LDL) or ‘bad’ cholesterol (which may put people at risk for heart disease and other serious conditions), and high-density lipoprotein (HDL) or ‘good’ cholesterol (which helps get rid of LDL).

p-value

The result of a statistical test which tells us whether the results of a study are likely to be due to chance and would not be confirmed if the study was repeated. All p-values are between 0 and 1; the most reliable studies have p-values very close to 0. A p-value of 0.001 means that there is a 1 in 1000 probability that the results are due to chance and do not reflect a real difference. A p-value of 0.05 means there is a 1 in 20 probability that the results are due to chance. When a p-value is 0.05 or below, the result is considered to be ‘statistically significant’. Confidence intervals give similar information to p-values but are easier to interpret. 

lipid

Fat or fat-like substances found in the blood and body tissues. Lipids serve as building blocks for cells and as a source of energy for the body. Cholesterol and triglycerides are types of lipids.

Mean plasma concentrations of lopinavir were 7.16µ/ml at baseline. 6.73µ/ml at week four, and 6.10µ/ml at week twelve. Triglyceride levels at baseline, weeks four and week twelve were 168mg/dL, 256mg/dL, and 276mg/dL respectively, and the increases in triglyceride levels at week four (p=0.006) and twelve (p=0.005) were significant. The investigators found a mean increase in triglyceride levels of 8.5-mg/dL for each week of follow-up (p=0.014).

However, no significant increase was seen in mean cholesterol levels throughout the period of follow-up.

Adjusting for time, the investigators failed to find any association between plasma concentrations of lopinavir and total triglyceride or cholesterol levels. What’s more, no association was found between an elevation in lopinavir levels of above 8µ/ml and grade three hyperlipidaemia (triglycerides above 750mg/dL and cholesterol above 300mg/dL), even though 40% of patients reached this cut-off concentration of lopinavir.

A total of six patients had grade two hyperlipidaemia (triglycerides above 400mg/dL and cholesterol above 240mg/dL) but none of these instances was related to plasma concentrations of lopinavir.

In a final analysis, the investigators looked at AUC values for lopinavir concentrations and failed to find any significant relationship with either triglyceride (p=0.092) or cholesterol (p=0.49).

”These results may indicate that it is premature to suggest a LPV/r dose reduction based on plasma drug concentrations to prevent or treat abnormal lipid elevations”, conclude the investigators.

Further information on this website

Kaletra - overview

Metabolic changes on HAART - overview

Factsheets on lipodystrophy

References

Torti C et al. Lipid abnormalities in HIV-infected patients are not correlated with lopinavir plasma concentrations. JAIDS 35: 324 -326, 2004.