Is HIV drug resistance spreading? Early warning signals say 'yes'

The PharmAccess research looked at 13 sites in 11 countries.
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Signals warning of the transmission of drug-resistant HIV are growing in low- and middle-income countries, and governments should step up surveillance efforts as they scale up treatment, experts concluded today at the Eighteenth Conference on Retroviruses and Opportunistic Infections (CROI) in Boston.

A World Health Organization (WHO) survey of 'early warning indicators' – levels of performance that treatment services should be hitting in order to minimise the risk of drug resistance – showed substantial problems with drug stock-outs, loss to follow-up and patients picking up drugs on time in nine African countries in 2008.

Research by PharmAccess, a Dutch foundation that provides HIV treatment services for the private sector in sub-Saharan Africa, shows that levels of transmitted drug resistance across eleven countries showed that the risk of identifying transmitted drug resistance increased by 38% for each year that a country had been scaling up antiretroviral treatment.

Glossary

drug resistance

A drug-resistant HIV strain is one which is less susceptible to the effects of one or more anti-HIV drugs because of an accumulation of HIV mutations in its genotype. Resistance can be the result of a poor adherence to treatment or of transmission of an already resistant virus.

sample

Studies aim to give information that will be applicable to a large group of people (e.g. adults with diagnosed HIV in the UK). Because it is impractical to conduct a study with such a large group, only a sub-group (a sample) takes part in a study. This isn’t a problem as long as the characteristics of the sample are similar to those of the wider group (e.g. in terms of age, gender, CD4 count and years since diagnosis).

nucleoside

A precursor to a building block of DNA or RNA. Nucleosides must be chemically changed into nucleotides before they can be used to make DNA or RNA. 

first-line therapy

The regimen used when starting treatment for the first time.

reverse transcriptase

A retroviral enzyme which converts genetic material from RNA into DNA, an essential step in the lifecycle of HIV. Several classes of anti-HIV drugs interfere with this stage of HIV’s life cycle: nucleoside reverse transcriptase inhibitors and nucleotide reverse transcriptase inhibitors (NRTIs) and non-nucleoside reverse transcriptase inhibitors (NNRTIs). 

Furthermore, a survey of recently infected young people in Kampala, Uganda, showed that 8.6% had evidence of drug-resistant virus, with resistance to all three classes of antiretroviral drug currently available in the country detected among the sample.

In Latin America, rates of transmitted drug resistance may run as high as 20% in some parts of Brazil, and 6.8% in Mexico, researchers reported.

Transmission of drug resistance

Viruses resistant to drugs used in HIV treatment may be transmitted by people on treatment that is failing, or by people who have stopped or interrupted treatment. The best way of preventing the transmission of drug-resistant virus, apart from consistent condom use during sex, is to ensure that doses of medication are never missed.

However, interruptions in drug supply and difficulties in reaching the clinic to pick up drugs, often due to poverty or fluctuating income, mean that even patients with good adherence to treatment may be blocked in their efforts to take medication consistently.

For this reason the World Health Organization developed a set of early warning indicators that are being monitored at a large sample of HIV clinics in sub-Saharan Africa, in order to give advance notice of problems, and to provide feedback to clinics on how they are doing.

Early warning indicators

A review of clinic performance in sub-Saharan Africa in 2008 found that in respect to indicators most likely to affect adherence, clinics needed to improve substantially to minimise the risk of resistance.

Early warning indicators

Survey of 130 clinics in 9 countries, 2008

Indicator

Proportion of clinics reaching target

100% prescription of WHO first-line recommended regimens

86%

< 20% loss to follow-up

43%

>70% retention on appropriate first-line regimen

42%

>90% of patients picking up prescribed ARVs on time

17%

>80% of clinic appointments attended as scheduled

55%

100% of drugs available at pharmacy at all times

42%

PharmAccess carried out a review of drug resistance in 2478 treatment-naive patients commencing antiretroviral treatment at 13 sites in eleven countries, and found an overall prevalence of 5.71% (95% confidence interval 4.82 to 6.70%). Resistance to all three drug classes was found (NRTIs 2.2%, NNRTIs 3.41% and PIs 1.27%). The presence of resistance at a site was significantly associated with the time since the initiation of ART provision in that geographic area (odds ratio 1.56, p<0.001). The researchers calculated that the risk of resistance increased by 38% for each year of ART provision.

A survey conducted in 2009 and 2010 in Kampala, Uganda, which sought to identify young people recently infected through voluntary counselling and testing centres, found an apparent increase in transmitted drug resistance when compared to a sample of young women tested through antenatal clinics in 2007. Although the level of resistance identified was somewhat higher than the 2007 sample, at 8.6%, the researchers said that the prevalence of resistance was still low to moderate.

Nevertheless, they say it is time to step up resistance surveillance in Uganda.

Resistance surveillance in Brazil using dried blood spot sampling and testing through a central laboratory showed large regional variations, with prevalence of transmitted resistance as high as 19.9% in Salvador province and 12.8% in Rio de Janeiro.

Unlike in Africa, where the predominant form of transmitted drug resistance is non-nucleoside reverse transcriptase inhibitor resistance relating to use of nevirapine and efavirenz, resistance in Brazil is more likely to be related to use of nucleoside analogues, and may reflect the past use of unboosted protease inhibitors, leading to greater emergence of NRTI resistance when those regimens failed.

Abstracts and webcast

You can view the abstracts and poster presentations from this research on the official conference website:

Abstract 622: www.retroconference.org/2011/Abstracts/42581.htm

Abstract 621: www.retroconference.org/2011/Abstracts/40687.htm

Abstract 624: www.retroconference.org/2011/Abstracts/40884.htm

Abstract 626: www.retroconference.org/2011/Abstracts/42062.htm

You can also watch a webcast of the presentations made at this conference session.

Webcast from Is HIV drug resistance spreading?.

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References

Hamers R et al. HIV-1 drug resistance in ARV-naïve individuals in sub-Saharan Africa is associated with time since scale-up of ART. Eighteenth Conference on Retroviruses and Opportunistic Infections, Boston, abstract 622, 2011.

Ndembi N et al. Increasing primary HIV-1 drug resistance among recently infected persons in Uganda, East Africa. Eighteenth Conference on Retroviruses and Opportunistic Infections, Boston, abstract 621, 2011.

Soares C et al. Prevalence of transmitted HIV-1 antiretroviral resistance among patients initiating ART in Brazil: a surveillance using dried blood spots. Eighteenth Conference on Retroviruses and Opportunistic Infections, Boston, abstract 624, 2011.

Jordan M et al. Monitoring ART: clinic and program performance using WHO HIV drug resistance early warning indicators in 21 countries. Eighteenth Conference on Retroviruses and Opportunistic Infections, Boston, abstract 626, 2011.